Screening for high-yielding Pichia pastoris clones: the production of G protein-coupled receptors as a case study.

File Description SizeFormat 
Chapter 7 final.docAccepted version255 kBMicrosoft WordDownload
Title: Screening for high-yielding Pichia pastoris clones: the production of G protein-coupled receptors as a case study.
Author(s): Singh, S
Gras, A
Fiez-Vandal, C
Martinez, M
Wagner, R
Byrne, B
Item Type: Journal Article
Abstract: Pichia pastoris is an established host for the production of a wide range of recombinant proteins including membrane proteins. The system has a particularly good track record for the production of G protein-coupled receptors (GPCRs). Generation and screening of expression clones with this system use standard molecular biology techniques. Multiple clones can be generated and screened in a matter of a few weeks making this similar to Escherichia coli in terms of speed. In addition, basic buffer components and the lack of expensive equipment make small-scale expression screening in P. pastoris very cost-effective. Here we describe the procedures used for small-scale GPCR production screening. © 2012 Springer Science+business Media, LLC.
Publication Date: 23-Apr-2012
URI: http://hdl.handle.net/10044/1/10021
DOI: http://dx.doi.org/10.1007/978-1-61779-770-5_7
ISSN: 1064-3745
Publisher: Springer
Start Page: 65
End Page: 73
Journal / Book Title: Methods Mol Biol
Volume: 866
Copyright Statement: © Springer-Verlag 2012. The original publication is available at www.springerlink.com
Conference Place: United States
Appears in Collections:Molecular Biosciences



Items in Spiral are protected by copyright, with all rights reserved, unless otherwise indicated.

Creative Commons