Acoustic wave sparsely-activated localization microscopy (AWSALM): super-resolution ultrasound imaging using acoustic activation and deactivation of nanodroplets

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Title: Acoustic wave sparsely-activated localization microscopy (AWSALM): super-resolution ultrasound imaging using acoustic activation and deactivation of nanodroplets
Authors: Zhang, G
Harput, S
Lin, S
Christensen-Jeffries, K
Leow, CH
Brown, J
Dunsby, C
Eckersley, R
Tang, M
Item Type: Journal Article
Abstract: Photo-activated localization microscopy (PALM) has revolutionized the field of fluorescence microscopy by breaking the diffraction limit in spatial resolution. In this study, “acoustic wave sparsely activated localization microscopy (AWSALM),” an acoustic counterpart of PALM, is developed to super-resolve structures which cannot be resolved by conventional B-mode imaging. AWSALM utilizes acoustic waves to sparsely and stochastically activate decafluorobutane nanodroplets by acoustic vaporization and to simultaneously deactivate the existing vaporized nanodroplets via acoustic destruction. In this method, activation, imaging, and deactivation are all performed using acoustic waves. Experimental results show that sub-wavelength micro-structures not resolvable by standard B-mode ultrasound images can be separated by AWSALM. This technique is flow independent and does not require a low concentration of contrast agents, as is required by current ultrasound super resolution techniques. Acoustic activation and deactivation can be controlled by adjusting the acoustic pressure, which remains well within the FDA approved safety range. In conclusion, this study shows the promise of a flow and contrast agent concentration independent super-resolution ultrasound technique which has potential to be faster and go beyond vascular imaging.
Issue Date: 2-Jul-2018
Date of Acceptance: 9-May-2018
URI: http://hdl.handle.net/10044/1/59994
DOI: https://dx.doi.org/10.1063/1.5029874
ISSN: 1077-3118
Publisher: AIP Publishing
Journal / Book Title: Applied Physics Letters
Volume: 113
Copyright Statement: ©2018 Author(s). All article content, exceptwhere otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Sponsor/Funder: Engineering & Physical Science Research Council (EPSRC)
Engineering & Physical Science Research Council (EPSRC)
Cancer Research UK
Funder's Grant Number: EP/M011933/1
EP/N015487/1
22353
Keywords: Science & Technology
Physical Sciences
Physics, Applied
Physics
IN-VIVO
DIFFRACTION-LIMIT
VAPORIZATION
09 Engineering
02 Physical Sciences
Applied Physics
Publication Status: Published
Article Number: 014101
Online Publication Date: 2018-07-02
Appears in Collections:Faculty of Engineering
Physics
Bioengineering
Photonics
Faculty of Natural Sciences



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