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A Bioinspired 3D micro-structure for graphene-based bacteria sensing

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Title: A Bioinspired 3D micro-structure for graphene-based bacteria sensing
Authors: Li, B
Tan, H
Anastasova-Ivanova, S
Power, M
Seichepine, F
Yang, G-Z
Item Type: Journal Article
Abstract: Nature is a great source of inspiration for the development of solutions for biomedical problems. We present a novel biosensor design utilizing two-photon polymerisation and graphene to fabricate an enhanced biosensing platform for the detection of motile bacteria. A cage comprising venous valve-inspired directional micro-structure is fabricated around graphene-based sensing electronics. The asymmetric 3D micro-structure promotes motile cells to swim from outside the cage towards the inner-most chamber, resulting in concentrated bacteria surrounding the central sensing region, thus enhancing the sensing signal. The concentrating effect is proved across a range of cell cultures - from 101 CFU/ml to 109 CFU/ml. Fluorescence analysis shows a 3.38–3.5 times enhanced signal. pH sensor presents a 2.14–3.08 times enhancement via the detection of cellar metabolite. Electrical measurements demonstrate an 8.8–26.7 times enhanced current. The proposed platform provides a new way of leveraging bio-inspired 3D printing and 2D materials for the development of sensing devices for biomedical applications.
Issue Date: 1-Jan-2019
Date of Acceptance: 26-Sep-2018
URI: http://hdl.handle.net/10044/1/65140
DOI: https://dx.doi.org/10.1016/j.bios.2018.09.087
ISSN: 0956-5663
Publisher: Elsevier
Start Page: 77
End Page: 84
Journal / Book Title: Biosensors and Bioelectronics
Volume: 123
Copyright Statement: © 2018 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/BY/4.0/).
Sponsor/Funder: Engineering & Physical Science Research Council (EPSRC)
Engineering & Physical Science Research Council (EPSRC)
Funder's Grant Number: EP/L014149/1
EP/P012779/1
Keywords: Science & Technology
Life Sciences & Biomedicine
Physical Sciences
Biophysics
Biotechnology & Applied Microbiology
Chemistry, Analytical
Electrochemistry
Nanoscience & Nanotechnology
Chemistry
Science & Technology - Other Topics
Graphene
Label-free bacteria sensing
3D printing
Microfabrication
Bio-inspired device
ESCHERICHIA-COLI
BIOSENSORS
FABRICATION
TRANSISTOR
VERSATILE
CELLS
OXIDE
ION
0301 Analytical Chemistry
0903 Biomedical Engineering
1007 Nanotechnology
Bioinformatics
Publication Status: Published
Online Publication Date: 2018-09-27
Appears in Collections:Faculty of Engineering
Computing



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