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Analysis of HLA-G expression in renal tissue in lupus nephritis: a pilot study

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Title: Analysis of HLA-G expression in renal tissue in lupus nephritis: a pilot study
Authors: Foschi, V
Bortolotti, D
Doyle, AF
Stratigou, V
Stephens, L
Trivedi, P
Rinaldi, R
Padovan, M
Bortoluzzi, A
Lightstone, L
Cairns, TD
Botto, M
Cook, TH
Rizzo, R
Govoni, M
Pickering, MC
Item Type: Journal Article
Abstract: BACKGROUND: The study aimed to investigate whether HLA-G antigen is expressed in the kidneys of patients affected by lupus nephritis (LN) and whether its detection in renal biopsies could be adopted as a marker of treatment response and prognosis. METHODS: Thirty renal biopsies from patients with LN were selected and analyzed through immunohistochemistry. Laboratory and clinical data were retrospectively collected at baseline, 6 and 12 months and at the latest clinical appointment. A number of patients (63.3%) were treated with rituximab (RTX) +/- methylprednisolone in the induction phase. The expression of HLA-G in glomeruli, tubules and infiltrating cells was examined and compared between lupus patients who achieved either complete or partial renal response and those who did not respond to treatment. RESULTS: HLA-G staining was observed in the glomeruli of 20 of 30 samples from patients with LN. The expression of the antigen was detected in podocytes, along glomerular capillary walls, on parietal glomerular epithelial cells and within the juxtaglomerular apparatus. Seventy per cent of patients whose glomeruli expressed HLA-G achieved partial or complete response at 6 months and 75% at the latest available follow up compared with 30% and 40%, respectively, of those who did not show any expression. The pattern of staining in tubules and infiltrating cells was highly variable precluding any clinical correlation. CONCLUSION: This study demonstrates that HLA-G is expressed in renal tissue in LN. Our retrospective data suggest that its expression could correlate with response to treatment.
Issue Date: 10-Jul-2019
Date of Acceptance: 10-Jun-2019
URI: http://hdl.handle.net/10044/1/71466
DOI: 10.1177/0961203319860582
ISSN: 1477-0962
Publisher: SAGE
Start Page: 1091
End Page: 1100
Journal / Book Title: Lupus
Volume: 28
Issue: 9
Copyright Statement: © 2019 The Authors. Article reuse guidelines: sagepub.com/journals-permissions. The final, definitive version of this paper has been published in Foschi, V., Bortolotti, D., Doyle, A. F., Stratigou, V., Stephens, L., Trivedi, P., … Pickering, M. C. (2019). Analysis of HLA-G expression in renal tissue in lupus nephritis: a pilot study. Lupus, 28(9), 1091–1100 by Sage Publications Ltd. All rights reserved. It is available at: https://doi.org/10.1177/0961203319860582
Sponsor/Funder: Wellcome Trust
Funder's Grant Number: 098476/Z/12/Z
Keywords: Science & Technology
Life Sciences & Biomedicine
Rheumatology
Systemic lupus erythematosus
HLA-G
lupus nephritis
rituximab
immunohistochemistry
LEUKOCYTE ANTIGEN-G
PARIETAL EPITHELIAL-CELLS
MESENCHYMAL STEM-CELLS
CLASS-I
INSERTION/DELETION POLYMORPHISM
PODOCYTE REGENERATION
G TRANSCRIPTS
SHLA-G
SUSCEPTIBILITY
ERYTHEMATOSUS
HLA-G
Systemic lupus erythematosus
immunohistochemistry
lupus nephritis
rituximab
HLA-G
Systemic lupus erythematosus
immunohistochemistry
lupus nephritis
rituximab
Arthritis & Rheumatology
1103 Clinical Sciences
Publication Status: Published
Conference Place: England
Online Publication Date: 2019-07-10
Appears in Collections:Department of Immunology and Inflammation