Line scanning, fiber bundle fluorescence HiLo endomicroscopy with confocal slit detection

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Title: Line scanning, fiber bundle fluorescence HiLo endomicroscopy with confocal slit detection
Authors: Zhang, H
Khushi, V
Yang, G-Z
Item Type: Journal Article
Abstract: Fiber bundle fluorescence endomicroscopy is an effective method for in vivo imaging of biological tissue samples. Line-scanning confocal laser endomicroscopy realizes confocal imaging at a much higher frame rate compared to the point scanning system, but with reduced optical sectioning. To address this problem, we describe a fiber bundle endomicroscopy system that utilizes the HiLo technique to enhance the optical sectioning while still maintaining high image acquisition rates. Confocal HiLo endomicroscopy is achieved by synchronizing the scanning hybrid-illumination laser line with the rolling shutter of a CMOS camera. An evident improvement of axial sectioning is achieved as compared to the line-scanning confocal endomicroscopy without the HiLo technique. Comparisons are also made with epifluorescence endomicroscopy with and without HiLo. The optical sectioning enhancement is demonstrated on lens tissue as well as porcine kidney tissue
Issue Date: 13-Nov-2019
Date of Acceptance: 7-Oct-2019
DOI: 10.1117/1.JBO.24.11.116501
ISSN: 1083-3668
Publisher: Society of Photo-optical Instrumentation Engineers
Journal / Book Title: Journal of Biomedical Optics
Volume: 24
Issue: 11
Copyright Statement: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI. [DOI: 10.1117/1.JBO.24.11.116501]
Sponsor/Funder: Engineering & Physical Science Research Council (EPSRC)
Funder's Grant Number: EP/N019318/1
Keywords: biomedical imaging
optical microscopy
spatial light modulators
0205 Optical Physics
1113 Opthalmology and Optometry
0903 Biomedical Engineering
Publication Status: Published
Article Number: ARTN 116501
Online Publication Date: 2019-11-13
Appears in Collections:Faculty of Engineering

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