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16S rRNA assessment of the influence of shading on early-successional biofilms in experimental streams

Title: 16S rRNA assessment of the influence of shading on early-successional biofilms in experimental streams
Authors: Lehmann, K
Singer, A
Bowes, MJ
Ings, NL
Field, D
Bell, T
Item Type: Journal Article
Abstract: Elevated nutrient levels can lead to excessive biofilm growth, but reducing nutrient pollution is often challenging. There is therefore interest in developing control measures for biofilm growth in nutrient-rich rivers that could act as complement to direct reductions in nutrient load. Shading of rivers is one option that can mitigate blooms, but few studies have experimentally examined the differences in biofilm communities grown under shaded and unshaded conditions. We investigated the assembly and diversity of biofilm communities using in situ mesocosms within the River Thames (UK). Biofilm composition was surveyed by 454 sequencing of 16S amplicons (∼400 bp length covering regions V6/V7). The results confirm the importance of sunlight for biofilm community assembly; a resource that was utilized by a relatively small number of dominant taxa, leading to significantly less diversity than in shaded communities. These differences between unshaded and shaded treatments were either because of differences in resource utilization or loss of diatom-structures as habitats for bacteria. We observed more co-occurrence patterns and network interactions in the shaded communities. This lends further support to the proposal that increased river shading can help mitigate the effects from macronutrient pollution in rivers.
Issue Date: 1-Dec-2015
Date of Acceptance: 19-Oct-2015
URI: http://hdl.handle.net/10044/1/77719
DOI: 10.1093/femsec/fiv129
ISSN: 0168-6496
Publisher: Oxford University Press (OUP)
Start Page: 1
End Page: 11
Journal / Book Title: FEMS Microbiology Ecology
Volume: 91
Issue: 12
Copyright Statement: © FEMS 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
Sponsor/Funder: The Royal Society
Funder's Grant Number: UF090325
Keywords: Science & Technology
Life Sciences & Biomedicine
Microbiology
biofilm diversity
riparian shading
biofilm composition
networks
RIPARIAN VEGETATION
LIGHT LIMITATION
WATER
PHOSPHORUS
RIVER
NUTRIENTS
COMMUNITY
PHYTOPLANKTON
PERIPHYTON
ALGAE
biofilm composition
biofilm diversity
networks
riparian shading
Bacteria
Base Sequence
Biofilms
Diatoms
Ecosystem
England
Eutrophication
Molecular Typing
RNA, Ribosomal, 16S
Rivers
Sequence Analysis, RNA
Sunlight
Water Microbiology
Diatoms
Bacteria
Biofilms
RNA, Ribosomal, 16S
Sequence Analysis, RNA
Water Microbiology
Ecosystem
Sunlight
Rivers
Eutrophication
Base Sequence
England
Molecular Typing
Science & Technology
Life Sciences & Biomedicine
Microbiology
biofilm diversity
riparian shading
biofilm composition
networks
RIPARIAN VEGETATION
LIGHT LIMITATION
WATER
PHOSPHORUS
RIVER
NUTRIENTS
COMMUNITY
PHYTOPLANKTON
PERIPHYTON
ALGAE
Microbiology
05 Environmental Sciences
06 Biological Sciences
11 Medical and Health Sciences
Publication Status: Published
Article Number: ARTN fiv129
Online Publication Date: 2015-10-23
Appears in Collections:Faculty of Natural Sciences
Grantham Institute for Climate Change